
Also called: Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test
Report
{context.blood_test.parameters} Parameters
What it checks
Report delivery
Test for
Age group
Commonly advised for
The 25-Hydroxy Vitamin D3 test measures the primary circulating form of vitamin D in the body. Vitamin D is essential for calcium absorption, bone health, immune function, and muscle strength.
Deficiency is extremely common and can lead to bone disorders, muscle pain, and increased risk of infections.
Why Is This Test Recommended?
This test is recommended to assess vitamin D deficiency, evaluate bone pain or weakness, monitor supplementation therapy, and screen high-risk individuals.
Understanding Vitamin D3
25-Hydroxy Vitamin D reflects both dietary intake and sun exposure. It is the most accurate marker of vitamin D status in the body and helps guide supplementation and treatment decisions.
Symptoms or Conditions That May Require This Test
Bone pain or tenderness
Muscle weakness or cramps
Frequent infections
Osteoporosis or osteopenia
Fatigue
Why Do I Need This Test?
To prevent bone loss, maintain overall health, and identify deficiency that may contribute to various health issues.
How Do I Prepare for the Test?
No fasting is required. You can take the test at any time of day.
How Is the Test Performed?
A blood sample is collected from a vein in your arm.
What Happens After the Test?
Vitamin D supplementation may be prescribed if levels are low, with dosage based on the severity of deficiency.
When Should I Consult a Doctor?
If your vitamin D levels are below normal range or if you experience symptoms of deficiency.
Risks Associated With This Test
Minimal risk related to blood draw only.
Lifestyle Tips
Get adequate sunlight exposure (10-15 minutes daily)
Include vitamin D-rich foods like fatty fish, egg yolks, and fortified dairy
Consider supplements if deficient
Maintain healthy weight
Regular outdoor activities
What Does This Test Measure?
The test measures vitamin D status in the body by detecting 25-hydroxy vitamin D levels.
What Do the Results Mean?
Low levels indicate vitamin D deficiency. Normal levels suggest adequate vitamin D status. Very high levels may indicate excessive supplementation.
What Should I Do If Results Are Abnormal?
Follow your doctor's prescribed supplementation plan and retest after treatment to monitor improvement.
Factors That May Affect Results
Sun exposure, dietary intake, age, skin pigmentation, obesity, malabsorption disorders, and certain medications.
Frequently Asked Questions (FAQs)
Is vitamin D deficiency common?
Yes, vitamin D deficiency is extremely common worldwide, affecting people across all age groups due to limited sun exposure and dietary intake.
Is sunlight enough?
Often not; while sunlight helps produce vitamin D, factors like geography, season, skin tone, and lifestyle often make supplementation necessary.
Can excess vitamin D be harmful?
Yes, excessive vitamin D from over-supplementation can cause toxicity, leading to high calcium levels, kidney problems, and other health issues.
Is fasting required?
No, fasting is not required for the 25-Hydroxy Vitamin D3 test.
How often should it be tested?
As advised by your doctor, typically every 3-6 months during treatment, then annually for monitoring once levels normalize.
This is the active form of vitamin D, tested in specific medical situations. It is advised if:
Tapadia Diagnostic Centre in Ahmedabad offers NABL-accredited Vitamin D (1,25-Dihydroxyvitamin D) test from a Blood sample(s). Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test is also prescribed for Bone pain, muscle weakness, muscle aches. Established in 1989, this doctor-led diagnostic centre ensures high accuracy. Home sample collection and walk-in facility available at our Ahmedabad center. Pay at the centre with no advance payment. Book online or visit us.
Vitamin D (1,25-Dihydroxyvitamin D) in Ahmedabad
Rated 4.6 on Google
From 6,477 patient reviews across our Hyderabad centres.
Every report, kept for you
Past reports stay in your account and can be downloaded again.
NABL accredited
Every result reviewed by a qualified pathologist before it reaches you.
Four decades of practice
A family-run diagnostic centre, trusted by doctors and households since 1989


Vitamin D (1,25-Dihydroxyvitamin D) in Vijayawada
Vitamin D (1,25-Dihydroxyvitamin D) in Visakhapatnam
Vitamin D (1,25-Dihydroxyvitamin D) in Ahmedabad
Vitamin D (1,25-Dihydroxyvitamin D) in Bengaluru
Vitamin D (1,25-Dihydroxyvitamin D) in Mumbai
Vitamin D (1,25-Dihydroxyvitamin D) in Secunderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Karimnagar
Vitamin D (1,25-Dihydroxyvitamin D) in Hyderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Warangal
Vitamin D (1,25-Dihydroxyvitamin D) in Aurangabad
HIV 1 & 2 Antibodies, Serum-Elisa Method in Ahmedabad
Dengue IgG, IgM Antibodies + NS1 Antigen-Rapid, Serum in Ahmedabad
Lipoprotein A (Lp A), Serum in Ahmedabad
Erythrocyte Sedimentation Rate (ESR) in Ahmedabad
Thyroxine Total (T4), Serum in Ahmedabad
Triiodothyronine Total (T3), Serum in Ahmedabad
CA 19.9 (Pancreatic Cancer Marker), Serum in Ahmedabad
NIPT with Microdeletions in Ahmedabad
NIPT Basic in Ahmedabad
Complete Blood Picture (CBP) in Ahmedabad
Serum Phosphorus Test in Ahmedabad
TIBC in Ahmedabad
Urology Profile in Ahmedabad
Beta HCG Free, Serum (Pregnancy Test) in Ahmedabad
Viral Screening in Ahmedabad
AFP Test in Ahmedabad
Microalbuminuria test in Ahmedabad
Diabetes Testing (FBS + PLBS) in Ahmedabad
Antithrombin III in Ahmedabad
D-dimer test in Ahmedabad
Creatine Kinase (CK) Test in Ahmedabad
AST Test in Ahmedabad
Cardiac Markers in Ahmedabad
Pap Smear - LBC in Ahmedabad
DVT Profile in Ahmedabad
Bleeding Time and Clotting Time (BT & CT) in Ahmedabad
Estradiol Test in Ahmedabad
hs-CRP Test in Ahmedabad
Alkaline Phosphatase (ALP) Test in Ahmedabad
Fertility Profile in Ahmedabad
Albumin blood test in Ahmedabad
Troponin T in Ahmedabad
Infertility Profile Extended in Ahmedabad
Electrolyte Panel in Ahmedabad
Anticardiolipin Antibodies (IgG) in Ahmedabad
PSA Test in Ahmedabad
FSH Test in Ahmedabad
STD Panel in Ahmedabad
PCOS Profile in Ahmedabad
Serum Iron Test in Ahmedabad
Cardiac Profile-III in Ahmedabad
Plasma Fibrinogen Test in Ahmedabad
HBsAg Test in Ahmedabad
Cardiac Biomarker Test in Ahmedabad
Herpes Simplex Virus (HSV) Types 1 and 2 Antibody Test in Ahmedabad
Antenatal Profile in Ahmedabad
Activated Partial Thromboplastin Time in Ahmedabad
Lipid Panel in Ahmedabad
Fine Needle Aspiration Cytology in Ahmedabad
CRP test in Ahmedabad
Joint Pain / SLE / Collagen Profile-I in Ahmedabad
PAPP-A Test in Ahmedabad
Insulin Test in Ahmedabad
Triple Screen Test in Ahmedabad
Anti-HCV Test in Ahmedabad
Basic Metabolic Panel (BMP) in Ahmedabad
Very low-density lipoprotein (VLDL) test in Ahmedabad
Free Testosterone in Ahmedabad
Triglycerides Test in Ahmedabad
Quad Screen in Ahmedabad
Folic Acid in Ahmedabad
Urinalysis in Ahmedabad
CEA Test in Ahmedabad
Ferritin Test in Ahmedabad
Transferrin Saturation% in Ahmedabad
Protein C Antigen in Ahmedabad
Alpha Feto Protein (AFP) - Fluid in Ahmedabad
CPK (MB) Test in Ahmedabad
Lupus Anticoagulant Testing in Ahmedabad
Reticulocyte Count in Ahmedabad
Infertility Profile-Basic in Ahmedabad
Beta hCG Test in Ahmedabad
DIABETIC PROFILE in Ahmedabad
LH Test in Ahmedabad
Anti-Müllerian Hormone (AMH) Test in Ahmedabad
Bile Salts and Bile Pigments, Urine in Ahmedabad
HbA1c in Ahmedabad
Vitamin D (1,25-Dihydroxyvitamin D) in Ahmedabad
Liver Function Test (LFT) in Ahmedabad
Anemia Profile Basic in Ahmedabad
Rheumatoid Factor in Ahmedabad
Widal Test in Ahmedabad
Bilirubin blood test in Ahmedabad
Inhibin A, Tumor Marker, Serum in Ahmedabad
Thyroid Function Tests in Ahmedabad
eGFR in Ahmedabad
Menopause Profile in Ahmedabad
EXTENDED DIABETIC PROFILE in Ahmedabad
LDH Test in Ahmedabad
Total Cholesterol Test in Ahmedabad
Random Blood Sugar Test in Ahmedabad
Total Protein Test in Ahmedabad
RPR test in Ahmedabad
Anti-dsDNA Test in Ahmedabad
HDL Cholesterol in Ahmedabad
Blood Type Test in Ahmedabad
ASO Titre in Ahmedabad
Blood Urea(Serum) Test in Ahmedabad
Postprandial Blood Sugar Test in Ahmedabad
Back Pain Profile in Ahmedabad
ANA test in Ahmedabad
Serum Creatinine Test in Ahmedabad
PT/INR Test in Ahmedabad
Fasting Blood Sugar Test in Ahmedabad
Extended Anemia Profile III Executive in Ahmedabad
Kidney Function Test (Renal Profile) in Ahmedabad
Serum Magnesium Test in Ahmedabad
Well-woman exam in Ahmedabad
LDL cholesterol test in Ahmedabad
Prolactin Test in Ahmedabad
Malaria Blood Smear in Ahmedabad
Liver Function Test (+GGT) in Ahmedabad
TPHA Test in Ahmedabad
Hepatitis B Surface Antigen (HBsAg) Test in Ahmedabad
Antenatal Profile 2 in Ahmedabad
TSH Test in Ahmedabad
Cardiac Catheterization in Ahmedabad
Cardiolipin Antibody (IgM) in Ahmedabad
Master Health Checkup in Ahmedabad
Calcium Profile in Ahmedabad
Coagulation Profile in Ahmedabad
Uric Acid Test in Ahmedabad
Vitamin D (25 - Hydroxy Vitamin D, Serum) in Ahmedabad
Globulin Test in Ahmedabad
Fever Profile Test in Ahmedabad
Connective Tissue Disease Profile in Ahmedabad
Calcium Blood Test in Ahmedabad
IHC in Ahmedabad
Estriol Unconjugated (E3) Test in Ahmedabad
Blood Typing in Ahmedabad
Vitamin B12 Test in Ahmedabad
Serum Cortisol in Ahmedabad
Double Marker Test in Ahmedabad
Pap Smear, Conventional in Ahmedabad
Protein S Activity in Ahmedabad
Non-Invasive Prenatal Testing (NIPT) in Ahmedabad
whole exome sequencing in Ahmedabad
Newborn Screening (NBS) in Ahmedabad
Renal Function Test (RFT) in Ahmedabad