
Also called: Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test
Report
{context.blood_test.parameters} Parameters
What it checks
Report delivery
Test for
Age group
Commonly advised for
The 25-Hydroxy Vitamin D3 test measures the primary circulating form of vitamin D in the body. Vitamin D is essential for calcium absorption, bone health, immune function, and muscle strength.
Deficiency is extremely common and can lead to bone disorders, muscle pain, and increased risk of infections.
Why Is This Test Recommended?
This test is recommended to assess vitamin D deficiency, evaluate bone pain or weakness, monitor supplementation therapy, and screen high-risk individuals.
Understanding Vitamin D3
25-Hydroxy Vitamin D reflects both dietary intake and sun exposure. It is the most accurate marker of vitamin D status in the body and helps guide supplementation and treatment decisions.
Symptoms or Conditions That May Require This Test
Bone pain or tenderness
Muscle weakness or cramps
Frequent infections
Osteoporosis or osteopenia
Fatigue
Why Do I Need This Test?
To prevent bone loss, maintain overall health, and identify deficiency that may contribute to various health issues.
How Do I Prepare for the Test?
No fasting is required. You can take the test at any time of day.
How Is the Test Performed?
A blood sample is collected from a vein in your arm.
What Happens After the Test?
Vitamin D supplementation may be prescribed if levels are low, with dosage based on the severity of deficiency.
When Should I Consult a Doctor?
If your vitamin D levels are below normal range or if you experience symptoms of deficiency.
Risks Associated With This Test
Minimal risk related to blood draw only.
Lifestyle Tips
Get adequate sunlight exposure (10-15 minutes daily)
Include vitamin D-rich foods like fatty fish, egg yolks, and fortified dairy
Consider supplements if deficient
Maintain healthy weight
Regular outdoor activities
What Does This Test Measure?
The test measures vitamin D status in the body by detecting 25-hydroxy vitamin D levels.
What Do the Results Mean?
Low levels indicate vitamin D deficiency. Normal levels suggest adequate vitamin D status. Very high levels may indicate excessive supplementation.
What Should I Do If Results Are Abnormal?
Follow your doctor's prescribed supplementation plan and retest after treatment to monitor improvement.
Factors That May Affect Results
Sun exposure, dietary intake, age, skin pigmentation, obesity, malabsorption disorders, and certain medications.
Frequently Asked Questions (FAQs)
Is vitamin D deficiency common?
Yes, vitamin D deficiency is extremely common worldwide, affecting people across all age groups due to limited sun exposure and dietary intake.
Is sunlight enough?
Often not; while sunlight helps produce vitamin D, factors like geography, season, skin tone, and lifestyle often make supplementation necessary.
Can excess vitamin D be harmful?
Yes, excessive vitamin D from over-supplementation can cause toxicity, leading to high calcium levels, kidney problems, and other health issues.
Is fasting required?
No, fasting is not required for the 25-Hydroxy Vitamin D3 test.
How often should it be tested?
As advised by your doctor, typically every 3-6 months during treatment, then annually for monitoring once levels normalize.
This is the active form of vitamin D, tested in specific medical situations. It is advised if:
Tapadia Diagnostic Centre in Guwahati offers NABL-accredited Vitamin D (1,25-Dihydroxyvitamin D) test from a Blood sample(s). Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test is also prescribed for Bone pain, muscle weakness, muscle aches. Established in 1989, this doctor-led diagnostic centre ensures high accuracy. Home sample collection and walk-in facility available at our Guwahati center. Pay at the centre with no advance payment. Book online or visit us.
Vitamin D (1,25-Dihydroxyvitamin D) in Guwahati
Rated 4.6 on Google
From 6,477 patient reviews across our Hyderabad centres.
Every report, kept for you
Past reports stay in your account and can be downloaded again.
NABL accredited
Every result reviewed by a qualified pathologist before it reaches you.
Four decades of practice
A family-run diagnostic centre, trusted by doctors and households since 1989


Vitamin D (1,25-Dihydroxyvitamin D) in Vijayawada
Vitamin D (1,25-Dihydroxyvitamin D) in Visakhapatnam
Vitamin D (1,25-Dihydroxyvitamin D) in Ahmedabad
Vitamin D (1,25-Dihydroxyvitamin D) in Bengaluru
Vitamin D (1,25-Dihydroxyvitamin D) in Mumbai
Vitamin D (1,25-Dihydroxyvitamin D) in Secunderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Karimnagar
Vitamin D (1,25-Dihydroxyvitamin D) in Hyderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Warangal
Vitamin D (1,25-Dihydroxyvitamin D) in Chhatrapati Sambhajinagar (Aurangabad)
Master Health Checkup (Blood) in Guwahati
HIV 1 & 2 Antibodies, Serum-Elisa Method in Guwahati
Dengue IgG, IgM Antibodies + NS1 Antigen-Rapid, Serum in Guwahati
Lipoprotein A (Lp A), Serum in Guwahati
Erythrocyte Sedimentation Rate (ESR) in Guwahati
Thyroxine Total (T4), Serum in Guwahati
Triiodothyronine Total (T3), Serum in Guwahati
CA 19.9 (Pancreatic Cancer Marker), Serum in Guwahati
NIPT with Microdeletions in Guwahati
NIPT Basic in Guwahati
Complete Blood Picture (CBP) in Guwahati
Serum Phosphorus Test in Guwahati
TIBC in Guwahati
Urology Profile in Guwahati
Beta HCG Free, Serum (Pregnancy Test) in Guwahati
Viral Screening in Guwahati
AFP Test in Guwahati
Microalbuminuria test in Guwahati
Diabetes Testing (FBS + PLBS) in Guwahati
Antithrombin III in Guwahati
D-dimer test in Guwahati
Creatine Kinase (CK) Test in Guwahati
AST Test in Guwahati
Cardiac Markers in Guwahati
Pap Smear - LBC in Guwahati
DVT Profile in Guwahati
Bleeding Time and Clotting Time (BT & CT) in Guwahati
Estradiol Test in Guwahati
hs-CRP Test in Guwahati
Alkaline Phosphatase (ALP) Test in Guwahati
Fertility Profile in Guwahati
Albumin blood test in Guwahati
Troponin T in Guwahati
Infertility Profile Extended in Guwahati
Electrolyte Panel in Guwahati
Anticardiolipin Antibodies (IgG) in Guwahati
PSA Test in Guwahati
FSH Test in Guwahati
STD Panel in Guwahati
PCOS Profile in Guwahati
Serum Iron Test in Guwahati
Cardiac Profile-III in Guwahati
Plasma Fibrinogen Test in Guwahati
HBsAg Test in Guwahati
Cardiac Biomarker Test in Guwahati
Herpes Simplex Virus (HSV) Types 1 and 2 Antibody Test in Guwahati
Antenatal Profile in Guwahati
Activated Partial Thromboplastin Time in Guwahati
Lipid Panel in Guwahati
Fine Needle Aspiration Cytology in Guwahati
CRP test in Guwahati
Joint Pain / SLE / Collagen Profile-I in Guwahati
PAPP-A Test in Guwahati
Insulin Test in Guwahati
Triple Screen Test in Guwahati
Anti-HCV Test in Guwahati
Basic Metabolic Panel (BMP) in Guwahati
Very low-density lipoprotein (VLDL) test in Guwahati
Free Testosterone in Guwahati
Triglycerides Test in Guwahati
Quad Screen in Guwahati
Folic Acid in Guwahati
Urinalysis in Guwahati
CEA Test in Guwahati
Ferritin Test in Guwahati
Transferrin Saturation% in Guwahati
Protein C Antigen in Guwahati
Alpha Feto Protein (AFP) - Fluid in Guwahati
CPK (MB) Test in Guwahati
Lupus Anticoagulant Testing in Guwahati
Reticulocyte Count in Guwahati
Infertility Profile-Basic in Guwahati
Beta hCG Test in Guwahati
DIABETIC PROFILE in Guwahati
LH Test in Guwahati
Anti-Müllerian Hormone (AMH) Test in Guwahati
Bile Salts and Bile Pigments, Urine in Guwahati
HbA1c in Guwahati
Vitamin D (1,25-Dihydroxyvitamin D) in Guwahati
Liver Function Test (LFT) in Guwahati
Anemia Profile Basic in Guwahati
Rheumatoid Factor in Guwahati
Widal Test in Guwahati
Bilirubin blood test in Guwahati
Inhibin A, Tumor Marker, Serum in Guwahati
Thyroid Function Tests in Guwahati
eGFR in Guwahati
Menopause Profile in Guwahati
EXTENDED DIABETIC PROFILE in Guwahati
LDH Test in Guwahati
Total Cholesterol Test in Guwahati
Random Blood Sugar Test in Guwahati
Total Protein Test in Guwahati
RPR test in Guwahati
Anti-dsDNA Test in Guwahati
HDL Cholesterol in Guwahati
Blood Type Test in Guwahati
ASO Titre in Guwahati
Blood Urea(Serum) Test in Guwahati
Postprandial Blood Sugar Test in Guwahati
Back Pain Profile in Guwahati
ANA test in Guwahati
Serum Creatinine Test in Guwahati
PT/INR Test in Guwahati
Fasting Blood Sugar Test in Guwahati
Extended Anemia Profile III Executive in Guwahati
Kidney Function Test (Renal Profile) in Guwahati
Serum Magnesium Test in Guwahati
Well-woman exam in Guwahati
LDL cholesterol test in Guwahati
Prolactin Test in Guwahati
Malaria Blood Smear in Guwahati
Liver Function Test (+GGT) in Guwahati
TPHA Test in Guwahati
Hepatitis B Surface Antigen (HBsAg) Test in Guwahati
Antenatal Profile 2 in Guwahati
TSH Test in Guwahati
Cardiac Catheterization in Guwahati
Cardiolipin Antibody (IgM) in Guwahati
Master Health Checkup (BL+RADIO) in Guwahati
Calcium Profile in Guwahati
Coagulation Profile in Guwahati
Uric Acid Test in Guwahati
Vitamin D (25 - Hydroxy Vitamin D, Serum) in Guwahati
Globulin Test in Guwahati
Fever Profile Test in Guwahati
Connective Tissue Disease Profile in Guwahati
Calcium Blood Test in Guwahati
IHC in Guwahati
Estriol Unconjugated (E3) Test in Guwahati
Blood Typing in Guwahati
Vitamin B12 Test in Guwahati
Serum Cortisol in Guwahati
Double Marker Test in Guwahati
Pap Smear, Conventional in Guwahati
Protein S Activity in Guwahati
Non-Invasive Prenatal Testing (NIPT) in Guwahati
whole exome sequencing in Guwahati
Newborn Screening (NBS) in Guwahati
Apolipoprotein A1 (Apo A1), Serum in Guwahati
Apolipoprotein B (Apo B), Serum in Guwahati
Renal Function Test (RFT) in Guwahati