
Also called: Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test
Report
{context.blood_test.parameters} Parameters
What it checks
Report delivery
Test for
Age group
Commonly advised for
The 25-Hydroxy Vitamin D3 test measures the primary circulating form of vitamin D in the body. Vitamin D is essential for calcium absorption, bone health, immune function, and muscle strength.
Deficiency is extremely common and can lead to bone disorders, muscle pain, and increased risk of infections.
Why Is This Test Recommended?
This test is recommended to assess vitamin D deficiency, evaluate bone pain or weakness, monitor supplementation therapy, and screen high-risk individuals.
Understanding Vitamin D3
25-Hydroxy Vitamin D reflects both dietary intake and sun exposure. It is the most accurate marker of vitamin D status in the body and helps guide supplementation and treatment decisions.
Symptoms or Conditions That May Require This Test
Bone pain or tenderness
Muscle weakness or cramps
Frequent infections
Osteoporosis or osteopenia
Fatigue
Why Do I Need This Test?
To prevent bone loss, maintain overall health, and identify deficiency that may contribute to various health issues.
How Do I Prepare for the Test?
No fasting is required. You can take the test at any time of day.
How Is the Test Performed?
A blood sample is collected from a vein in your arm.
What Happens After the Test?
Vitamin D supplementation may be prescribed if levels are low, with dosage based on the severity of deficiency.
When Should I Consult a Doctor?
If your vitamin D levels are below normal range or if you experience symptoms of deficiency.
Risks Associated With This Test
Minimal risk related to blood draw only.
Lifestyle Tips
Get adequate sunlight exposure (10-15 minutes daily)
Include vitamin D-rich foods like fatty fish, egg yolks, and fortified dairy
Consider supplements if deficient
Maintain healthy weight
Regular outdoor activities
What Does This Test Measure?
The test measures vitamin D status in the body by detecting 25-hydroxy vitamin D levels.
What Do the Results Mean?
Low levels indicate vitamin D deficiency. Normal levels suggest adequate vitamin D status. Very high levels may indicate excessive supplementation.
What Should I Do If Results Are Abnormal?
Follow your doctor's prescribed supplementation plan and retest after treatment to monitor improvement.
Factors That May Affect Results
Sun exposure, dietary intake, age, skin pigmentation, obesity, malabsorption disorders, and certain medications.
Frequently Asked Questions (FAQs)
Is vitamin D deficiency common?
Yes, vitamin D deficiency is extremely common worldwide, affecting people across all age groups due to limited sun exposure and dietary intake.
Is sunlight enough?
Often not; while sunlight helps produce vitamin D, factors like geography, season, skin tone, and lifestyle often make supplementation necessary.
Can excess vitamin D be harmful?
Yes, excessive vitamin D from over-supplementation can cause toxicity, leading to high calcium levels, kidney problems, and other health issues.
Is fasting required?
No, fasting is not required for the 25-Hydroxy Vitamin D3 test.
How often should it be tested?
As advised by your doctor, typically every 3-6 months during treatment, then annually for monitoring once levels normalize.
This is the active form of vitamin D, tested in specific medical situations. It is advised if:
Tapadia Diagnostic Centre in Pune offers NABL-accredited Vitamin D (1,25-Dihydroxyvitamin D) test from a Blood sample(s). Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test is also prescribed for Bone pain, muscle weakness, muscle aches. Established in 1989, this doctor-led diagnostic centre ensures high accuracy. Home sample collection and walk-in facility available at our Pune center. Pay at the centre with no advance payment. Book online or visit us.
Vitamin D (1,25-Dihydroxyvitamin D) in Pune
Rated 4.6 on Google
From 6,477 patient reviews across our Hyderabad centres.
Every report, kept for you
Past reports stay in your account and can be downloaded again.
NABL accredited
Every result reviewed by a qualified pathologist before it reaches you.
Four decades of practice
A family-run diagnostic centre, trusted by doctors and households since 1989


Vitamin D (1,25-Dihydroxyvitamin D) in Vijayawada
Vitamin D (1,25-Dihydroxyvitamin D) in Visakhapatnam
Vitamin D (1,25-Dihydroxyvitamin D) in Ahmedabad
Vitamin D (1,25-Dihydroxyvitamin D) in Bengaluru
Vitamin D (1,25-Dihydroxyvitamin D) in Mumbai
Vitamin D (1,25-Dihydroxyvitamin D) in Secunderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Karimnagar
Vitamin D (1,25-Dihydroxyvitamin D) in Hyderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Warangal
Vitamin D (1,25-Dihydroxyvitamin D) in Chhatrapati Sambhajinagar (Aurangabad)
Master Health Checkup (Blood) in Pune
HIV 1 & 2 Antibodies, Serum-Elisa Method in Pune
Dengue IgG, IgM Antibodies + NS1 Antigen-Rapid, Serum in Pune
Lipoprotein A (Lp A), Serum in Pune
Erythrocyte Sedimentation Rate (ESR) in Pune
Thyroxine Total (T4), Serum in Pune
Triiodothyronine Total (T3), Serum in Pune
CA 19.9 (Pancreatic Cancer Marker), Serum in Pune
NIPT with Microdeletions in Pune
NIPT Basic in Pune
Complete Blood Picture (CBP) in Pune
Serum Phosphorus Test in Pune
TIBC in Pune
Urology Profile in Pune
Beta HCG Free, Serum (Pregnancy Test) in Pune
Viral Screening in Pune
AFP Test in Pune
Microalbuminuria test in Pune
Diabetes Testing (FBS + PLBS) in Pune
Antithrombin III in Pune
D-dimer test in Pune
Creatine Kinase (CK) Test in Pune
AST Test in Pune
Cardiac Markers in Pune
Pap Smear - LBC in Pune
DVT Profile in Pune
Bleeding Time and Clotting Time (BT & CT) in Pune
Estradiol Test in Pune
hs-CRP Test in Pune
Alkaline Phosphatase (ALP) Test in Pune
Fertility Profile in Pune
Albumin blood test in Pune
Troponin T in Pune
Infertility Profile Extended in Pune
Electrolyte Panel in Pune
Anticardiolipin Antibodies (IgG) in Pune
PSA Test in Pune
FSH Test in Pune
STD Panel in Pune
PCOS Profile in Pune
Serum Iron Test in Pune
Cardiac Profile-III in Pune
Plasma Fibrinogen Test in Pune
HBsAg Test in Pune
Cardiac Biomarker Test in Pune
Herpes Simplex Virus (HSV) Types 1 and 2 Antibody Test in Pune
Antenatal Profile in Pune
Activated Partial Thromboplastin Time in Pune
Lipid Panel in Pune
Fine Needle Aspiration Cytology in Pune
CRP test in Pune
Joint Pain / SLE / Collagen Profile-I in Pune
PAPP-A Test in Pune
Insulin Test in Pune
Triple Screen Test in Pune
Anti-HCV Test in Pune
Basic Metabolic Panel (BMP) in Pune
Very low-density lipoprotein (VLDL) test in Pune
Free Testosterone in Pune
Triglycerides Test in Pune
Quad Screen in Pune
Folic Acid in Pune
Urinalysis in Pune
CEA Test in Pune
Ferritin Test in Pune
Transferrin Saturation% in Pune
Protein C Antigen in Pune
Alpha Feto Protein (AFP) - Fluid in Pune
CPK (MB) Test in Pune
Lupus Anticoagulant Testing in Pune
Reticulocyte Count in Pune
Infertility Profile-Basic in Pune
Beta hCG Test in Pune
DIABETIC PROFILE in Pune
LH Test in Pune
Anti-Müllerian Hormone (AMH) Test in Pune
Bile Salts and Bile Pigments, Urine in Pune
HbA1c in Pune
Vitamin D (1,25-Dihydroxyvitamin D) in Pune
Liver Function Test (LFT) in Pune
Anemia Profile Basic in Pune
Rheumatoid Factor in Pune
Widal Test in Pune
Bilirubin blood test in Pune
Inhibin A, Tumor Marker, Serum in Pune
Thyroid Function Tests in Pune
eGFR in Pune
Menopause Profile in Pune
EXTENDED DIABETIC PROFILE in Pune
LDH Test in Pune
Total Cholesterol Test in Pune
Random Blood Sugar Test in Pune
Total Protein Test in Pune
RPR test in Pune
Anti-dsDNA Test in Pune
HDL Cholesterol in Pune
Blood Type Test in Pune
ASO Titre in Pune
Blood Urea(Serum) Test in Pune
Postprandial Blood Sugar Test in Pune
Back Pain Profile in Pune
ANA test in Pune
Serum Creatinine Test in Pune
PT/INR Test in Pune
Fasting Blood Sugar Test in Pune
Extended Anemia Profile III Executive in Pune
Kidney Function Test (Renal Profile) in Pune
Serum Magnesium Test in Pune
Well-woman exam in Pune
LDL cholesterol test in Pune
Prolactin Test in Pune
Malaria Blood Smear in Pune
Liver Function Test (+GGT) in Pune
TPHA Test in Pune
Hepatitis B Surface Antigen (HBsAg) Test in Pune
Antenatal Profile 2 in Pune
TSH Test in Pune
Cardiac Catheterization in Pune
Cardiolipin Antibody (IgM) in Pune
Master Health Checkup (BL+RADIO) in Pune
Calcium Profile in Pune
Coagulation Profile in Pune
Uric Acid Test in Pune
Vitamin D (25 - Hydroxy Vitamin D, Serum) in Pune
Globulin Test in Pune
Fever Profile Test in Pune
Connective Tissue Disease Profile in Pune
Calcium Blood Test in Pune
IHC in Pune
Estriol Unconjugated (E3) Test in Pune
Blood Typing in Pune
Vitamin B12 Test in Pune
Serum Cortisol in Pune
Double Marker Test in Pune
Pap Smear, Conventional in Pune
Protein S Activity in Pune
Non-Invasive Prenatal Testing (NIPT) in Pune
whole exome sequencing in Pune
Newborn Screening (NBS) in Pune
Apolipoprotein A1 (Apo A1), Serum in Pune
Apolipoprotein B (Apo B), Serum in Pune
Renal Function Test (RFT) in Pune