
Also called: Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test
Report
{context.blood_test.parameters} Parameters
What it checks
Report delivery
Test for
Age group
Commonly advised for
The 25-Hydroxy Vitamin D3 test measures the primary circulating form of vitamin D in the body. Vitamin D is essential for calcium absorption, bone health, immune function, and muscle strength.
Deficiency is extremely common and can lead to bone disorders, muscle pain, and increased risk of infections.
Why Is This Test Recommended?
This test is recommended to assess vitamin D deficiency, evaluate bone pain or weakness, monitor supplementation therapy, and screen high-risk individuals.
Understanding Vitamin D3
25-Hydroxy Vitamin D reflects both dietary intake and sun exposure. It is the most accurate marker of vitamin D status in the body and helps guide supplementation and treatment decisions.
Symptoms or Conditions That May Require This Test
Bone pain or tenderness
Muscle weakness or cramps
Frequent infections
Osteoporosis or osteopenia
Fatigue
Why Do I Need This Test?
To prevent bone loss, maintain overall health, and identify deficiency that may contribute to various health issues.
How Do I Prepare for the Test?
No fasting is required. You can take the test at any time of day.
How Is the Test Performed?
A blood sample is collected from a vein in your arm.
What Happens After the Test?
Vitamin D supplementation may be prescribed if levels are low, with dosage based on the severity of deficiency.
When Should I Consult a Doctor?
If your vitamin D levels are below normal range or if you experience symptoms of deficiency.
Risks Associated With This Test
Minimal risk related to blood draw only.
Lifestyle Tips
Get adequate sunlight exposure (10-15 minutes daily)
Include vitamin D-rich foods like fatty fish, egg yolks, and fortified dairy
Consider supplements if deficient
Maintain healthy weight
Regular outdoor activities
What Does This Test Measure?
The test measures vitamin D status in the body by detecting 25-hydroxy vitamin D levels.
What Do the Results Mean?
Low levels indicate vitamin D deficiency. Normal levels suggest adequate vitamin D status. Very high levels may indicate excessive supplementation.
What Should I Do If Results Are Abnormal?
Follow your doctor's prescribed supplementation plan and retest after treatment to monitor improvement.
Factors That May Affect Results
Sun exposure, dietary intake, age, skin pigmentation, obesity, malabsorption disorders, and certain medications.
Frequently Asked Questions (FAQs)
Is vitamin D deficiency common?
Yes, vitamin D deficiency is extremely common worldwide, affecting people across all age groups due to limited sun exposure and dietary intake.
Is sunlight enough?
Often not; while sunlight helps produce vitamin D, factors like geography, season, skin tone, and lifestyle often make supplementation necessary.
Can excess vitamin D be harmful?
Yes, excessive vitamin D from over-supplementation can cause toxicity, leading to high calcium levels, kidney problems, and other health issues.
Is fasting required?
No, fasting is not required for the 25-Hydroxy Vitamin D3 test.
How often should it be tested?
As advised by your doctor, typically every 3-6 months during treatment, then annually for monitoring once levels normalize.
This is the active form of vitamin D, tested in specific medical situations. It is advised if:
Tapadia Diagnostic Centre in Ranchi offers NABL-accredited Vitamin D (1,25-Dihydroxyvitamin D) test from a Blood sample(s). Vitamin D blood test, 25-hydroxyvitamin D test, 25(OH)D test is also prescribed for Bone pain, muscle weakness, muscle aches. Established in 1989, this doctor-led diagnostic centre ensures high accuracy. Home sample collection and walk-in facility available at our Ranchi center. Pay at the centre with no advance payment. Book online or visit us.
Vitamin D (1,25-Dihydroxyvitamin D) in Ranchi
Rated 4.6 on Google
From 6,477 patient reviews across our Hyderabad centres.
Every report, kept for you
Past reports stay in your account and can be downloaded again.
NABL accredited
Every result reviewed by a qualified pathologist before it reaches you.
Four decades of practice
A family-run diagnostic centre, trusted by doctors and households since 1989


Vitamin D (1,25-Dihydroxyvitamin D) in Vijayawada
Vitamin D (1,25-Dihydroxyvitamin D) in Visakhapatnam
Vitamin D (1,25-Dihydroxyvitamin D) in Ahmedabad
Vitamin D (1,25-Dihydroxyvitamin D) in Bengaluru
Vitamin D (1,25-Dihydroxyvitamin D) in Mumbai
Vitamin D (1,25-Dihydroxyvitamin D) in Secunderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Karimnagar
Vitamin D (1,25-Dihydroxyvitamin D) in Hyderabad
Vitamin D (1,25-Dihydroxyvitamin D) in Warangal
Vitamin D (1,25-Dihydroxyvitamin D) in Chhatrapati Sambhajinagar (Aurangabad)
Master Health Checkup (Blood) in Ranchi
HIV 1 & 2 Antibodies, Serum-Elisa Method in Ranchi
Dengue IgG, IgM Antibodies + NS1 Antigen-Rapid, Serum in Ranchi
Lipoprotein A (Lp A), Serum in Ranchi
Erythrocyte Sedimentation Rate (ESR) in Ranchi
Thyroxine Total (T4), Serum in Ranchi
Triiodothyronine Total (T3), Serum in Ranchi
CA 19.9 (Pancreatic Cancer Marker), Serum in Ranchi
NIPT with Microdeletions in Ranchi
NIPT Basic in Ranchi
Complete Blood Picture (CBP) in Ranchi
Serum Phosphorus Test in Ranchi
TIBC in Ranchi
Urology Profile in Ranchi
Beta HCG Free, Serum (Pregnancy Test) in Ranchi
Viral Screening in Ranchi
AFP Test in Ranchi
Microalbuminuria test in Ranchi
Diabetes Testing (FBS + PLBS) in Ranchi
Antithrombin III in Ranchi
D-dimer test in Ranchi
Creatine Kinase (CK) Test in Ranchi
AST Test in Ranchi
Cardiac Markers in Ranchi
Pap Smear - LBC in Ranchi
DVT Profile in Ranchi
Bleeding Time and Clotting Time (BT & CT) in Ranchi
Estradiol Test in Ranchi
hs-CRP Test in Ranchi
Alkaline Phosphatase (ALP) Test in Ranchi
Fertility Profile in Ranchi
Albumin blood test in Ranchi
Troponin T in Ranchi
Infertility Profile Extended in Ranchi
Electrolyte Panel in Ranchi
Anticardiolipin Antibodies (IgG) in Ranchi
PSA Test in Ranchi
FSH Test in Ranchi
STD Panel in Ranchi
PCOS Profile in Ranchi
Serum Iron Test in Ranchi
Cardiac Profile-III in Ranchi
Plasma Fibrinogen Test in Ranchi
HBsAg Test in Ranchi
Cardiac Biomarker Test in Ranchi
Herpes Simplex Virus (HSV) Types 1 and 2 Antibody Test in Ranchi
Antenatal Profile in Ranchi
Activated Partial Thromboplastin Time in Ranchi
Lipid Panel in Ranchi
Fine Needle Aspiration Cytology in Ranchi
CRP test in Ranchi
Joint Pain / SLE / Collagen Profile-I in Ranchi
PAPP-A Test in Ranchi
Insulin Test in Ranchi
Triple Screen Test in Ranchi
Anti-HCV Test in Ranchi
Basic Metabolic Panel (BMP) in Ranchi
Very low-density lipoprotein (VLDL) test in Ranchi
Free Testosterone in Ranchi
Triglycerides Test in Ranchi
Quad Screen in Ranchi
Folic Acid in Ranchi
Urinalysis in Ranchi
CEA Test in Ranchi
Ferritin Test in Ranchi
Transferrin Saturation% in Ranchi
Protein C Antigen in Ranchi
Alpha Feto Protein (AFP) - Fluid in Ranchi
CPK (MB) Test in Ranchi
Lupus Anticoagulant Testing in Ranchi
Reticulocyte Count in Ranchi
Infertility Profile-Basic in Ranchi
Beta hCG Test in Ranchi
DIABETIC PROFILE in Ranchi
LH Test in Ranchi
Anti-Müllerian Hormone (AMH) Test in Ranchi
Bile Salts and Bile Pigments, Urine in Ranchi
HbA1c in Ranchi
Vitamin D (1,25-Dihydroxyvitamin D) in Ranchi
Liver Function Test (LFT) in Ranchi
Anemia Profile Basic in Ranchi
Rheumatoid Factor in Ranchi
Widal Test in Ranchi
Bilirubin blood test in Ranchi
Inhibin A, Tumor Marker, Serum in Ranchi
Thyroid Function Tests in Ranchi
eGFR in Ranchi
Menopause Profile in Ranchi
EXTENDED DIABETIC PROFILE in Ranchi
LDH Test in Ranchi
Total Cholesterol Test in Ranchi
Random Blood Sugar Test in Ranchi
Total Protein Test in Ranchi
RPR test in Ranchi
Anti-dsDNA Test in Ranchi
HDL Cholesterol in Ranchi
Blood Type Test in Ranchi
ASO Titre in Ranchi
Blood Urea(Serum) Test in Ranchi
Postprandial Blood Sugar Test in Ranchi
Back Pain Profile in Ranchi
ANA test in Ranchi
Serum Creatinine Test in Ranchi
PT/INR Test in Ranchi
Fasting Blood Sugar Test in Ranchi
Extended Anemia Profile III Executive in Ranchi
Kidney Function Test (Renal Profile) in Ranchi
Serum Magnesium Test in Ranchi
Well-woman exam in Ranchi
LDL cholesterol test in Ranchi
Prolactin Test in Ranchi
Malaria Blood Smear in Ranchi
Liver Function Test (+GGT) in Ranchi
TPHA Test in Ranchi
Hepatitis B Surface Antigen (HBsAg) Test in Ranchi
Antenatal Profile 2 in Ranchi
TSH Test in Ranchi
Cardiac Catheterization in Ranchi
Cardiolipin Antibody (IgM) in Ranchi
Master Health Checkup (BL+RADIO) in Ranchi
Calcium Profile in Ranchi
Coagulation Profile in Ranchi
Uric Acid Test in Ranchi
Vitamin D (25 - Hydroxy Vitamin D, Serum) in Ranchi
Globulin Test in Ranchi
Fever Profile Test in Ranchi
Connective Tissue Disease Profile in Ranchi
Calcium Blood Test in Ranchi
IHC in Ranchi
Estriol Unconjugated (E3) Test in Ranchi
Blood Typing in Ranchi
Vitamin B12 Test in Ranchi
Serum Cortisol in Ranchi
Double Marker Test in Ranchi
Pap Smear, Conventional in Ranchi
Protein S Activity in Ranchi
Non-Invasive Prenatal Testing (NIPT) in Ranchi
whole exome sequencing in Ranchi
Newborn Screening (NBS) in Ranchi
Apolipoprotein A1 (Apo A1), Serum in Ranchi
Apolipoprotein B (Apo B), Serum in Ranchi
Renal Function Test (RFT) in Ranchi