Also called: Syphilis Antibody Test, Syphilis Blood Test, Syphilis Screening Test
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TPHA is a confirmatory blood test for syphilis, detecting antibodies against Treponema pallidum. It remains positive for life once infected, even after treatment.
Confirm syphilis infection
Antenatal screening
Blood donor screening
Neurological symptoms of unknown cause
It is used after a screening test like VDRL. A positive TPHA confirms exposure.
Does TPHA indicate active infection? Not always, as TPHA detects lifelong antibodies from past exposure rather than current bacterial activity, so additional tests like VDRL titers or dark-field microscopy are needed to distinguish active from resolved syphilis.
Can it become negative after treatment? No, TPHA typically remains positive for life after infection, even with successful antibiotic treatment, making it a reliable marker of lifetime exposure but not a gauge of cure.
Is fasting required? No, fasting is not required for the TPHA test, as it measures specific antibodies unaffected by recent food intake, allowing for convenient scheduling anytime.
Is it used alone? No, TPHA is not used alone but as a confirmatory test following initial screening like VDRL or RPR to verify true positives and avoid false alarms from non-specific reactions.
Is it reliable? Yes, TPHA is highly reliable with excellent specificity for detecting Treponema pallidum antibodies, making it a gold standard confirmatory test in syphilis diagnosis worldwide.
The TPHA test confirms syphilis after a screening test such as RPR. It is advised if:
Tapadia Diagnostic Centre in Secunderabad offers NABL-accredited TPHA Test test from a Blood sample(s). Syphilis Antibody Test, Syphilis Blood Test, Syphilis Screening Test is also prescribed for Painless sores, rash, fever, swollen glands. Established in 1989, this doctor-led diagnostic centre ensures high accuracy. Home sample collection and walk-in facility available at our Secunderabad center. Pay at the centre with no advance payment. Book online or visit us.
Sree Ranganatha Swamy Devasthanam, Yapral, H.No. 5-3-1, Bhagat Singh Nagar, Yapral
Services: Lab, ECG
Branch Incharge: Mr. Praveen Gupta : 9848056578
59/1/2(6-1R-002), Karkana,Secunderabad-09
Services: Lab, X Ray, ECG
Phone No: 9281063662
Branch Incharge: Mr. ASHOK : 9246846874
6-1-103, Ground Floor, Padma raonagar, Secunderabad.
Services: Lab, X Ray, ECG, U/S Scan
Phone No: 9390110059
Branch Incharge: Mr. Suman- 8179607955
TPHA Test in Secunderabad
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Every result reviewed by a qualified pathologist before it reaches you.
Four decades of practice
A family-run diagnostic centre, trusted by doctors and households since 1989


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